Glycine Increases Insulin Sensitivity and Glutathione Biosynthesis and Protects against Oxidative Stress in a Model of Sucrose-Induced Insulin Resistance
Table 2
Oxidative stress markers and glutathione (GSH) metabolism in rat liver.
Variables
C
CG
SF
SFG
GSH
45.5 ± 7.1
87.7 ± 7.9
17.4 ± 4.12
38.7 ± 8.4
GSSG
0.7 ± 0.3
0.87 ± 0.25
2.2 ± 0.8
1.17 ± 0.35
Cys
17.6 ± 4.6
20.0 ± 6.4
7.2 ± 3.4
23.3 ± 5.9
γ-GC
11.3 ± 2.7
17.7 ± 4.2
4.60 ± 2.1
12.9 ± 5.1
GSH/GSSG
105.6 ± 24.6
141 ± 32.5
8.91 ± 5.41
46.2 ± 23.5
Protein carbonyls (nmol/mg)
0.13 ± 0.03
0.33 ± 0.2
1.54 ± 0.8
0.6 ± 0.14
TBARs (nmol/mg)
10.4 ± 2.2
13.5 ± 3.5
23.0 ± 1.2
15.65 ± 3.5
Glycine
Plasma (μM)
234.6 ± 13.5
275.4 ± 24.5
205 ± 21.5
275.4 ± 15.7
Liver homogenate (nmol/mg protein)
655.7 ± 25.7
756.6 ± 35.4
584.3 ± 35.6
656.5 ± 37.5
Values represent mean ± SD, different animals. (C versus CG), (SF versus C), and (SFG versus SF). GSSG: oxidized glutathione; Cys: cysteine; γ-GC: γ-glutamylcysteine.
