Antiaging of Cucurbitane Glycosides from Fruits of Momordica charantia L.

Figure 4

Effect of compound 9 on the antioxidative ability of yeast cells and ROS level of yeast. (a) BY4741 yeast cells in the control group and compound 9-treated groups were dropped in the same YPGlucose agar plate mixed with 9 mM H₂O₂. After four days, the growth of yeast cells in different groups was photographed. (b) Effect of compound 9 on the survival rates of yeast under oxidative stress condition. Control, 48.31 ± 4.94; Res, 72.74 ± 6.19; compound 9 at 1 μM, 71.21 ± 2.75and at 3 μM, 68.73 ± 5.89. The experiment was conducted at least thrice. Vertical bars represent the mean ± SEM of three assays (). (c) The change of ROS level of yeast after administration compound 9 at 1, 3 μM. Control, 2.77 ± 0.15; Res, 1.79 ± 0.15; compound 9 at 1 μM, 1.98 ± 0.08, and at 3 μM, 1.73 ± 0.22. Vertical bars represent the mean ± SEM of 6 repeats ( and , compared with the control).

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