An In Vivo Zebrafish Model for Interrogating ROS-Mediated Pancreatic β-Cell Injury, Response, and Prevention

Figure 4

Antioxidant treatment protects from metronidazole-induced ROS generation in β-cells. Zebrafish larvae ( per condition) were treated with 5 mM metronidazole ± N-acetyl-L-cysteine (NAC) for 1, 6, or 24 hours followed by an assessment of ROS using CellROX green stain. (a) Representative images of islets of 106 hpf zebrafish (NTR⁺) embryos treated with 5 mM MTZ ± NAC at different time points. (b) Quantification of CellROX green intensity shows NAC-mediated protection from MTZ-induced ROS in β-cells after 1 or 6 hours of treatment. Data are presented as mean ± SEM (). Statistical significance was determined by Student’s t-test. Scale bar indicates 10 μm.

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