An In Vivo Zebrafish Model for Interrogating ROS-Mediated Pancreatic β-Cell Injury, Response, and Prevention

Figure 3

Metronidazole induces apoptosis signaling in β-cells. (a) Representative image of vehicle-treated zebrafish islets () after fixing at 106 hpf. (b) Representative image of islets of zebrafish (NTR⁺) larvae ( per condition) treated with 2.5 mM or 7.5 mM MTZ at different time points and immune-stained for insulin and cleaved caspase 3 (Casp3). (c) Quantification of Casp3 intensity shows a significant increase after 6 hours of treatment in the β-cells of 7.5 mM MTZ-treated embryos compared to vehicle. (d) Representative immunofluorescence images of zebrafish (mpeg+) islets (/condition) treated with 7.5 mM MTZ showing macrophage invasion into islets (green arrows) and their engulfment of β-cells (yellow arrows). Data are presented as mean ± SEM (). Statistical significance was determined by one-way ANOVA followed by post hoc Holm-Sidak test. Scale bar indicates 10 μm.

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