An In Vivo Zebrafish Model for Interrogating ROS-Mediated Pancreatic β-Cell Injury, Response, and Prevention

Figure 2

Metronidazole induces ROS generation in a dose-dependent manner. (a) Representative image of vehicle-treated zebrafish islets () at 106 hpf. (b) Representative image of islets of zebrafish (NTR⁺) larvae ( per condition) treated with 2.5 mM and 7.5 mM MTZ at different time points. (c) Quantification of CellROX intensity shows a significant increase after 1 or 6 hours of treatment in the β-cells of 7.5 mM MTZ-treated embryos, as compared to untreated controls. Data are presented as mean ± SEM (). Statistical significance was determined by one-way ANOVA followed by post hoc Holm-Sidak test. Scale bar indicates 10 μm.

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