Vialinin A, an Edible Mushroom-Derived p-Terphenyl Antioxidant, Prevents VEGF-Induced Neovascularization In Vitro and In Vivo
Table 1
Effect of vialinin A on VEGF-induced release of angiogenic cytokines in HUVECs.
C
VEGF
VIA
VIA + VEGF
Angiopoietin-2
54869.6 ± 434.8
66142.3 ± 1184.9
25800.6 ± 373.6
26516.8 ± 179.03##
BMP9
2.6 ± 0.1
2.5 ± 0.2
2.4 ± 0.1
2.7 ± 0.5
EGF
306.9 ± 10.7
315.9 ± 4.3
297.2 ± 7.3
341.1 ± 19.8
Endoglin
5496.3 ± 211.7
4560.4 ± 317.6
3600.6 ± 45.6
3970.9 ± 172.2
Endothelin-1
2304.1 ± 57.4
2320.2 ± 375.4
132.4 ± 7.6
107.7 ± 7.7##
FGF-1
15.005 ± 0.5
14.6 ± 0.4
14.4 ± 0.2
14.8 ± 0.5
FGF-2
794.9 ± 54.5
707.3 ± 41.1
659.1 ± 22.1
751.8 ± 34.9
Follistatin
113.1 ± 7.2
174.3 ± 9.7
123.5 ± 2.8
157.3 ± 3.8
G-CSF
79.2 ± 20.4
169.1 ± 63.3
62.9 ± 6.6
93.7 ± 45.3##
HB-EGF
522.6 ± 20.2
1437.8 ± 38.8
806.1 ± 25.4
1067.1 ± 40.2
HGF
145.6 ± 11.4
188.5 ± 24.2
80.5 ± 3.8
97.7 ± 3.9##
IL-8
2693.04 ± 198.8
3233.3 ± 41.3
3468.1 ± 14.4
3454.6 ± 24.7
PLGF
5344.4 ± 23.2
5105.4 ± 79.7
2909.3 ± 11.02
2655.4 ± 64.9
VEGF-C
1014.4 ± 43.5
1437.1 ± 116.4
231.6 ± 12.09
262.3 ± 11.6##
VEGF-D
4.6 ± 2.7
5.4 ± 2.1
4.3 ± 1.6
5.3 ± 3.4
Growth-arrested HUVECs were treated with vialinin A (5 μM) ± VEGF 10 ng/mL for 24 h. Angiogenic cytokines and chemokines were determined in the culture media using a human angiogenesis/growth factor magnetic bead panel kit from Millipore according to the manufacturer’s instructions using the Milliplex system. Data shown in pg/mL analyzed by xPONENT software. Values are the mean ± SD (). and compared to untreated control and ## compared to VEGF treated.
