Oleic Acid and Hydroxytyrosol Inhibit Cholesterol and Fatty Acid Synthesis in C6 Glioma Cells

Figure 5

Effect of oleic acid, hydroxytyrosol, or their combination on ACC, FAS, and HMGCR mRNA abundance and protein content in C6 glioma cells. C6 cells were incubated with 25 μM of OA, HTyr, or OA + HTyr for 4 h in serum-rich medium. (a) The abundance of ACC, FAS, and HMGCR mRNA was determined by RT-qPCR and normalized with respect to 18S rRNA, used as a reference. Normalized values are expressed in histograms as percentage of the control. Values are means ± S.D. of triplicate samples from each of four independent experiments. (b) C6 cells were lysed and protein content was isolated. ACC, FAS, and HMGCR were then assessed by Western blotting and quantified by densitometry. The protein contents are expressed as percentage of the control and are means ± SD of three independent experiments. Within the same group, samples bearing different letters differ significantly (). OA: oleic acid; HTyr: hydroxytyrosol.

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