Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture

Figure 8

Expression of autophagy marker Beclin-1 following zinc supplementation.(a) Representative Western blot from untreated control, oxPOS treated, 1.5 μM zinc, 15 μM zinc, 1.5 μM zinc + oxPOS treated, and oxPOS treated + 15 μM zinc-supplemented RPE cells. Bands were detected for Beclin-1 at 55 KDa and β-actin at 40 KDa. (b) Quantification of Beclin-1 protein expression by RPE cells in the different treatment groups. Signals from Western blots were visualised by Odyssey infrared imaging system and quantified using ImageJ analysis software (version 1.45). Signals for control were set to one and the data are represented as relative fold change, , compared to the untreated control group. One-way ANOVA followed by Dunnett’s multiple comparison test. .

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