Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture

Figure 5

Dose-dependent effect of zinc on 4-HNE-induced RPE cell death. RPE cells subjected to different treatments were fixed and processed for TUNEL assay. (a) Control RPE cells. (b) 5 μM 4-HNE only-treated RPE cells. (c) 1.5 μM zinc + 4-HNE-treated RPE cells. (d) 5 μM zinc + 4-HNE-treated RPE cells. (e) 15 μM zinc + 4-HNE-treated RPE cells. Red nuclei staining indicates TUNEL positive cells. (f) Histogram showing the percentage of TUNEL positive cells in each treatment groups, and , compared to control with data analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test. 100 cells were counted for each well. .

(a)

(b)

(c)

(d)

(e)

(f)