Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture

Figure 3

Changes in mitochondria morphology staining and function in a stressed environment. RPE was subjected to treatments with 1.5 μM zinc, 1.5 μM zinc + oxPOS treated, oxPOS treated + 15 μM zinc supplemented, and oxPOS-treated untreated control for 48 h. MitoTracker was added for 30 min at 37°C before imaging the cells living under the confocal microscope at 60x magnification or samples were processed for Mitochondrial ToxGlo assay to measure ATP production. (a) Control-untreated RPE cells. (b) oxPOS-treated RPE cells. (c) 1.5 μM zinc RPE cells. (d) 1.5 μM zinc + oxPOS-treated RPE cells. (e) 15 μM zinc-supplemented RPE cells. (f) Quantitative data of mitochondria ATP production in different treatment groups, and , compared to untreated control with data analyzed using a one-way ANOVA followed by Dunnett’s multiple comparison test. .

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