Spermidine Oxidation-Mediated Degeneration of Retinal Pigment Epithelium in Rats

Figure 1

Effects of serum removal and treatment with amine oxidase inhibitors, an antioxidant compound, and aldehyde dehydrogenase (ALDH) on spermidine-induced ARPE-19 cell death. Cell viability was assessed with a MTS assay 24 h after the addition of spermidine. The effects of (a) serum removal and treatment with (b) amine oxidase inhibitors, (c) an antioxidant, and (d) ALDH when applied simultaneously with spermidine. Each column represents a mean ± standard error of the mean of four wells. ^## versus control. versus spermidine. AG: aminoguanidine. PEN: pentamidine.

(a) FBS (+) versus FBS (−)

(b) Spermidine + AG or PEN

(c) Spermidine + NAC

(d) Spermidine + ALDH