NRF2 Plays a Critical Role in Both Self and EGCG Protection against Diabetic Testicular Damage

Figure 3

EGCG prevented diabetes-induced activation of testicular apoptotic cell death signaling via NRF2. Testicular apoptotic signaling was further evaluated by determining (a) the ratio of Bax protein level to Bcl2 protein level, along with the protein levels of (b) pro-caspase3 and (c) c-caspase3. To further assess the activity of caspase3, (d) the ratio of c-caspase3 to pro-caspase3 was calculated. For (b) and (c), the protein levels were normalized with GAPDH. Data were normalized as fold variation to Ctrl and were presented as means ± SD (). versus Ctrl; ^† versus DM. Bax: Bcl-2-associated X protein; Bcl-2: B-cell lymphoma 2; c-caspase3: cleaved caspase3; other abbreviations are the same as those in Figure 1.

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