Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach

Table 5

List of differentially expressed proteins identified by MS/MS in CTR and CTR-Res-treated samples.

Spot number	Swiss-Prot accession number	Protein name	Gene name	Mascot score		Sequence coverage MS	Sequence coverage MS/MS	Peptides	Fold change CTR-Res/CTR	value	Instrument
				PMF	MS/MS
Spot 1	P41250	Glicine-tRNA ligase (SYG)	GARS	91	144	21%	6%	K.NNIIQTWR.Q; R.SCYDLSCHAR.A; K.LPFAAAQIGNSFR.N; K.TSYGWIEIVGCCADR.S	−1.6		MALDI-TOF/TOF
Spot 2	Q9Y4L1	Hypoxia upregulated protein (HYOU1)	HYOU1	181	106	25%	2%	K.LCQGLFFR.V; K.QADNPHVALYQAR.F	−2.3		MALDI-TOF/TOF
Spot 3	O43707	Alpha-actinin 4 (ACTN4)	ACTN4	130	111	26%	3%	K.GYEEWLLNEIR.R; R.ASFNHFDKDHGGALGPEEFK.A	−1.9		MALDI-TOF/TOF
Spot 4	P55072	Transitional endoplasmic reticulum ATPase (TERA)	VCP	/	97	/	24%	R.GILLYGPPGTGK.T; R.EVDIGIPDATGR.L; R.WALSQSNPSALR.E; R.IVSQLLTLMDGLK.Q; R.LGDVISIQPCPDVK.Y; R.LDQLIYIPLPDEK.S; K.MTNGFSGADLTEICQR.A; K.NAPAIIFIDELDAIAPK.R; K.MTNGFSGADLTEICQR.A + oxidation (M); R.QAAPCVLFFDELDSIAK.A; R.EDEEESLNEVGYDDIGGCR.K; R.ETVVEVPQVTWEDIGGLEDVKR.E	−2.5		ESI-Trap
Spot 5	P18669	Phosphoglycerate mutase 1(PGAM1)	PGAM1	73	149	27%	8%	R.VLIAAHGNSLR.G; R.HGESSAWNLENR.F	1.7		MALDI-TOF/TOF
Spot 6	P60174	Triosephosphate isomerase (TPIS)	TPI1	112	175	42%	9%	R.HVFGESDELIGQK.V; K.DCGATWVVLGHSER.R	1.6		MALDI-TOF/TOF
Spot 7	Q06830	Peroxiredoxin-1 (PRDX1)	PRDX1	/	47	/	29%	R.TIAQDYGVLK.A; K.ATAVMPDGQFK.D + oxidation (M); R.LVQAFQFTDK.H; R.QITVNDLPVGR.S	−1.9		ESI-Trap
Spot 8	P06733	Alpha enolase (ENOA)	ENO1	145	162	51%	14%	R.YISPDQLADLYK,S; K.LAQANGWGVMVSHR.S + oxidation (M); R.AAVPSGASTGIYEALELR.D; K.LAMQEFMILPVGAANFR.E + 2 oxidation (M)	2.2		MALDI-TOF/TOF
Spot 9	P06733	Alpha enolase (ENOA)	ENO1	145	96	51%	8%	R.AAVPSGASTGIYEALELR.D; K.LAMQEFMILPVGAANFR.E + 2 oxidation (M)	2.4		MALDI-TOF/TOF
Spot 10	P02511	Alpha-crystallin B chain (CRYAB)	CRYAB	103	79	12%	11%	R.QDEHGFISR.E; R.RPFFPFHSPSR.L	1.6		MALDI-TOF/TOF
Spot 11	Q99497	DJ-1 (PARK7)	PARK7	88	44	50%	19%	K.DGLILTSR.G; K.MMNGGHYTYSENR.V + oxidation (M); K.GAEEMETVIPVDVMR.R	1.7		MALDI-TOF/TOF
Spot 12	Q14764	Major vault protein (MVP)	MVP	160	117	31%	3%	R.GAVASVTFDDFHK.N; R.IPPYHYIHVLDGNSNVSR.V	−1.6		MALDI-TOF/TOF
Spot 13	P02545	Prelamin A/C (LMNA)	LMNA	130	185	29%	5%	R.LADALQELR.A; R.TLEGELHDLR.G; R.NSNLVGAAHEELQQSR.I	1.8		MALDI-TOF/TOF
Spot 14	P30101	Protein disulfide-isomerase A3 (PDIA3)	PDIA3	107	59	23%	1%	K.QAGPASVPLR.T	−2.1		MALDI-TOF/TOF
Spot 15	P30086	Phosphatidylethanolamine-binding protein 1 (PEBP1)	PEBP1	187	238	67%	22%	K.LYTLVLTDPDAPSR.K; K.NRPTSISWDGLDSGK.L; R.YVWLVYEQDRPLK.C	2.2		MALDI-TOF/TOF
Spot 16	P32119	Peroxiredoxin-2 (PRDX2)	PRDX2	93	324	47%	14%	R.QITVNDLPVGR.S; K.EGGLGPLNIPLLADVTR.R; R.KEGGLGPLNIPLLADVTR.R	1.8		MALDI-TOF/TOF
Spot 18	P08670	Vimentin (VIME)	VIM	62	124	15%	13%	K.FADLSEAANRNNDALR.Q; R.QVQSLTCEVDALKGTNESLER.Q	−1.6		MALDI-TOF/TOF
Spot 19	P07237	Protein disulfide-isomerase (PDIA1)	P4HB	130	142	27%	7%	K.FFPASADR.T; K.VDATEESDLAQQYGVR.G; K.ILFIFIDSDHTDNQR.I	−2		MALDI-TOF/TOF
Spot 20	P62826	GTP-binding nuclear protein Ran	RAN	74	99	25%	8%	K.YVATLGVEVHPLVFHTNR.G; K.KYVATLGVEVHPLVFHTNR.G	1.8		MALDI-TOF/TOF

Spot numbers match those reported in the representative 2-DE images shown in Figure 2. Accession number in Swiss-Prot/UniprotKB (http://www.uniprot.org/). Fold change (resveratrol-treated cells versus control cells) was calculated dividing the average of %V resveratrol-treated cells by the average of %V control cells of three independent experiments. t-test was performed by GraphPad v4.0 software to determine if the relative change was statistically significant (); ; ; .