Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach

Figure 1

Representative 2-DE gel map of proteins isolated from human CTR and P1 fibroblasts. (a) A total of 80 μg of proteins were separated by 2-DE using a 13 cm IPG strip pH 3–10 NL and 12% SDS-PAGE. Proteins were visualized by silver staining. Spot numbers indicate proteins that were differentially regulated between CTR and P1 samples. (b, c) Bioinformatics analysis of differentially expressed proteins. (b) A high confidence protein-protein interaction network generated with STRING using our protein dataset is shown. The network nodes are input proteins. The edges represent the predicted functional associations. An edge may be drawn with up to 7 differently colored lines—these lines represent the existence of the seven types of evidence used in predicting the associations. A red line indicates the presence of fusion evidence; a green line, neighborhood evidence; a blue line, coocurrence evidence; a purple line, experimental evidence; a yellow line, textmining evidence; a light blue line, database evidence; and a black line, coexpression evidence. (c) Proteins involved in protein processing in the endoplasmic reticulum proteins are highlighted in red (HYOU1, GANAB, CALR, HSPA5, HSP90B1, VCP, and HSPA8) in the main PPI network.

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