The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 9

Mitochondrial ROS induced by ¹²⁵I seeds irradiation is critical for the expression of HIF-1α and its target gene. (a–c) HCT116 cells were exposed to 2 Gy of ¹²⁵I seeds radiation. Real-time PCR was performed to detect the expression of HIF-1α (a), BNIP3 (b), and NIX (c). (d–f) HCT116 cells were pretreated with and without 5 mM of NAC for 4 hours and then either mock exposed or exposed to 2 Gy of ¹²⁵I seeds radiation. The level of mRNA of HIF-1α (d), BNIP3 (e), and NIX (f) was detected by real-time PCR after treatment. (g–h) The irradiated HCT116 cells were treated with or without 50 uM of Antimycin A for 18 hours before harvest. The level of mRNA of HIF-1α (g), BNIP3 (h), and NIX (i) was detected by real-time PCR after treatment. indicates a significant difference () between NAC treated and untreated cells.

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