The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 4

¹²⁵I seeds radiation promoted autophagosome formation in HCT116 cells. (a) Irradiated HCT116 cells were treated with or without 40 mol of chloroquine for 6 hours. LC3 punctuations were detected using immunofluorescence and images were captured by a confocal microscope. (b) The percentage of LC3 punctuation per cell was quantified. The values are the means ± SD of at least three different cells. (c) HCT116 cells were exposed to 125I seeds radiation (2 Gy) with or without chloroquine for 6 hours, respectively. The ratio of LC3II/I was measured by Western blotting analysis. indicates significant difference () as compared to the control group.

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