The Protective Roles of ROS-Mediated Mitophagy on ¹²⁵I Seeds Radiation Induced Cell Death in HCT116 Cells

Figure 2

Inhibition of autophagy promoted apoptosis induced by ¹²⁵I seeds radiation. (a) Representative example of flow cytometry analysis pretreated with and without 3-MA before exposure to indicated doses of ¹²⁵I seeds radiation. Apoptotic cell death was detected by Annexin V and PI double staining. (b) The percentage of apoptosis with and without 3-MA was examined by flow cytometry after Annexin V and PI double staining. Annexin V⁺/PI⁻ cell and anexin V⁺/PI⁺ cell populations were quantified and analyzed. (c) Typical example of flow cytometry analysis pretreated with and without Ly294002 before exposure to indicated doses of ¹²⁵I seeds radiation. (d) The percentage of apoptosis with and without Ly294002 was measured by flow cytometry with Annexin V and PI double staining. Annexin V⁺/PI⁻ cell and anexin V⁺/PI⁺ cell populations were quantified and analyzed. (e) Typical example of flow cytometry analysis in HCT116 cells transfected with LC3 siRNA or unrelated control siRNA before exposure to the indicated dose of ¹²⁵I seeds radiation. (f) The percentage of apoptosis in HCT116 cells transfected with LC3 siRNA or unrelated control siRNA was measured by flow cytometry with Annexin V and PI double staining. Annexin V⁺/PI⁻ cell and anexin V⁺/PI⁺ cell populations were quantified and analyzed. indicates significant difference () between treated group or untreated group.

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