Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells

Figure 3

Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells. (a–d) IPEC-J2 cells were incubated with OEO 10 μg/mL for 3–24 h. (e–h) IPEC-J2 cells were incubated with OEO (2.5–10 μg/mL) for 12 h. Relative mRNA expression levels were measured quantitatively using real-time RT-PCR. The results were normalized to β-actin and expressed as fold increase over control. (i) IPEC-J2 cells were incubated with OEO (2.5–10 μg/mL) for 24 h. GCLC, GCLM, and SOD1 were estimated by western blot. (j) IPEC-J2 cells were incubated with OEO (2.5–10 μg/mL) for 24 h, and intracellular glutathione (GSH) content was determined. (k) IPEC-J2 cells were incubated with OEO (2.5–10 μg/mL) for 24 h, and intracellular SOD1 content was determined. Values represent means ± SEM, . , , and , compared with untreated control cells. Means without a common letter differ, .

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