Proteomics-Based Identification of the Molecular Signatures of Liver Tissues from Aged Rats following Eight Weeks of Medium-Intensity Exercise

Table 1

Proteins differentially expressed in aged rat livers after exercise training, determined using MALDI-TOF MS/MS.

Spot; number	GI; number	Sequence Cov. (%)	Measured Mr (kDa)/pI	MOWSE; score	Protein identification	Fold change
A2	8394158	7	275101/5.96	197	Fatty acid synthase (FAS)	−2.08
A8	132573	21	51653/4.67	77	Ribonuclease/angiogenin inhibitor 1 (RNASE1)	−2.04
A9	158186651	20	47440/6.16	347	α-Enolase (ENO1)	−2.30
A10	19924087	22	37517/6.67	76	Hydroxyprostaglandin dehydrogenase (11β-HSD1)	−2.64
A12	734703982	10	112398/5.99	52	Scaffold attachment factor B (SAFB)	−3.13
A13	149056650	38	28234/6.78	81	Ethylmalonic encephalopathy protein 1 (ETHE1)	−2.20
A16	54019419	28	25502/4.85	215	Proteasome subunit beta type-6 (PSMB6)	+2.85
B2	80861401	10	48828/6.08	204	T-kininogen	+2.32
B5	730311	18	61650/6.30	149	Glucose phosphomutase (PGM)	+2.60
B6	205057	39	48828/5.83	204	Keratin 8 (K8)	+2.94
B8	77157805	16	44240/5.61	139	S-Adenosylmethionine synthetase isoform type-1 (SAMs)	+2.55
B10	59808182	12	39052/6.10	146	Haptoglobin (HP)	+2.14

Theoretical pI and Mr derived from NCBInr and Swiss Prot/TrEML databases.
Score is −10 × log(), where is the probability that the observed match is a random event, based on the NCBInr database using the MASCOT searching program as MALDI-TOF MS/MS data (http://www.matrixscience.com/).