PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

Figure 3

PRMT1 or PRMT4 overexpression increases RPE cell apoptosis, while PRMT1 overexpression decreases SIRT1 expression. (a, b) ARPE-19 cells were transfected with HA or HA-PRMT1 plasmid DNA. After 36 h, (a) cell viability was measured by the MTT assay. The data represent the means ± SEM of three independent experiments, each performed in triplicate. versus HA. (b) Cell extracts were subjected to Western blotting with the indicated antibodies. The data represent the means ± SEM of three independent experiments. versus HA. (c, d) ARPE-19 cells were transfected with HA or HA-PRMT4 plasmid DNA. After 36 h, (c) cell viability was measured by the MTT assay. The data represent the means ± SEM of three independent experiments, each performed in triplicate. versus HA. (d) Cell extracts were subjected to Western blotting with the indicated antibodies. The data represent the means ± SEM of three independent experiments. versus HA (n.s. = nonspecific).

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