Nitric Oxide Regulates Neurogenesis in the Hippocampus following Seizures

Figure 4

The number of BrdU+ cells in the dentate gyrus increased following seizures, 21 days after BrdU treatment, by a NO-independent mechanism. (a) Representative images of the distribution of BrdU (green) and NeuN (red) positive cells in different layers of the dentate gyrus of the hippocampus, 3 days after induction of seizures with KA or saline treatment, in iNOS^+/+ and iNOS^−/− mice. Scale bar: 20 μm. (b) Number of BrdU+ cells in iNOS^+/+ and iNOS^−/− mice. Data are expressed as means ± SEM. Two-factor ANOVA (Bonferroni’s posttest): to 7; , significantly different from iNOS^+/+ saline; , significantly different from iNOS^−/− saline. (c) BrdU+ cells in different layers of the dentate gyrus, namely, in the SGZ, IGZ and OGZ of iNOS^+/+ vs iNOS^−/− mice, 3 days following SE. Data are expressed as means ± SEM. Two-factor ANOVA (Bonferroni’s posttest): to 7; , significantly different from iNOS^+/+ saline; , significantly different from iNOS^−/− saline.

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