BMP-2 Overexpression Augments Vascular Smooth Muscle Cell Motility by Upregulating Myosin Va via Erk Signaling

Table 1

(a) PCR primer sequences. (b) Information regarding antibodies employed in Western analysis.

(a)

Gene name	Sense	Antisense	Size (bp)
BMP-2	5′-TATATGCTCGACCTGTACCG-3′	5′-CTTCCTGCATTTGTTCCCGA-3′	247
Myosin Va	5′-CACCTACGGAACCCTGACAT-3′	5′-TGCAAAGATGTGAGGGTCCA-3′	243
Myosin Vb	5′-GGCTGAACTAACCAAGGACT-3′	5′-GGCAACAAGCACAATTCCAC-3′	256
Myosin Vc	5′-GAAGATGGAAGGAAAGCAGG-3′	5′-TCCTCAAGATCCCTGTTTTC-3′	255
SPP1	5′-TGGCTTACGGACTGAGGTCA-3′	5′-GACCTCAGAAGATGAACTC-3′	486
MGP	5′-ATCCTGGCTGCGCTGGCCGTG-3′	5′-GAAGTAGCGGTTGTAGGCGGC-3′	264
Sma22	5′-AAGCCAGTGAAGGTGCCTGAG-3′	5′-TTGAAGGCCAATCACGTGCTT-3′	311
Fstl1	5′-GTGGCAGTAATGGCAAGAC-3′	5′-GTACTTGTCTAGGATCTCAC-3′	260
GAPDH	5′-AAGAAGGTGGTGAAGCAGG-3′	5′-ACCCTGTTGCTGTAGCCATA-3′	197

(b)

Name	Vender	Cat. no.	Species	Dilution
BMP-2	Bioworld	BS3473	Rabbit monoclonal IgG	1 : 500
MYO5a	Sigma	Sab2501441	Goat polyclonal IgG	1 : 1000
Smad1	Cell Signaling	9516	Rabbit monoclonal IgG	1 : 1000
Sm22	Epitomics	S2112	Rabbit polyclonal IgG	1 : 1000
OPN	Bioworld	BS1264	Rabbit monoclonal IgG	1 : 1000
MGP	Abgent	Ap11953	Rabbit polyclonal IgG	1 : 200
FSTL1	Abcam	Ab71548	Rabbit polyclonal IgG	1 : 1000
Actin	Anbo	C0124	Rabbit polyclonal IgG	1 : 5000