Brazilian Green Propolis Suppresses the Hypoxia-Induced Neuroinflammatory Responses by Inhibiting NF-κB Activation in Microglia
Figure 3
Inhibitory effects of propolis on the hypoxia-induced NF-κB activation in cultured microglia. (a) Phosphorylation of IκBα in MG6 microglia exposed to normoxia (20% O2) or hypoxia (1% O2) in the presence or absence of propolis (50 μg/mL) for 24 h. (b) The quantitative analyses of immunoblots in (a). Each column and bar represent the mean ± SEM ( each). An asterisk indicates a statistically significant difference from the value in Normoxia (*). A sword indicates a statistically significant difference from the value in hypoxia (†). (c) Immunofluorescent CLMS images of p65 (green) with Hoechst-stained nuclei (blue) in MG6 microglia exposed to normoxia (20% O2) or hypoxia (1% O2) in the presence or absence of propolis (50 μg/mL) for 24 h.
