Astragalus Polysaccharide Suppresses Skeletal Muscle Myostatin Expression in Diabetes: Involvement of ROS-ERK and NF-κB Pathways
Figure 4
Effects of APS on the production of ROS in palmitate-stimulated C2C12 cells. C2C12 cells were incubated for 24 h in DMEM containing 5% BSA in either the presence (FFA-treated cells) or absence (control cells) of 0.5 mmol/L FFAs. Cells treated with APS were incubated with additional APS at the final concentration of 200 μg/mL. The intracellular levels of ROS were determined by fluorescence using a fluorescence microplate reader with excitation/emission set to 485/530 nm. Cells were plated at 1 × 106 cells/mL and pretreated with APS for 0.5 h, followed by incubation with palmitate for 24 h. Data are expressed as means ± SD of 6 different experiments. ### versus vehicle, *** versus palmitate. ROS: reactive oxygen species. PAL: palmitate.
