Astragalus Polysaccharide Suppresses Skeletal Muscle Myostatin Expression in Diabetes: Involvement of ROS-ERK and NF-κB Pathways
Figure 3
Effect of APS on glucose uptake and myostatin expression in C2C12 cells exposure to palmitate. C2C12 cells were incubated for 24 h in DMEM containing 5% BSA in either the presence (FFA-treated cells) or absence (control cells) of 0.5 mmol/L FFAs. Cells treated with APS were incubated with additional APS at the final concentration of 200 μg/mL. (a) Glucose uptake was assayed using [3H]2-deoxyglucose in the presence or in the absence of 100 nM insulin. # versus vehicle, * versus palmitate. (b) Myostatin protein expression was assessed by western blot. Data are expressed as means ± SD of 6 different experiments. ### versus vehicle, *** versus palmitate; PAL: palmitate; MSTN: myostatin.
