Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells
Figure 6
Effect of Nox4 inhibition on nuclear ROS production and DNA damage. (a) Representative images showing staining with nuclear ROS probe (Nuclear peroxy Emerald 1) of AFSC in the presence or absence of 2 μM plumbagin or 1 μM DPI for 24 hours. Scale bar: 10 μm. (b) Representative images showing superimposing between Nox4 SC (green) and PH2A (red) signals with or without DAPI (blue) of AFSC in the presence or absence of 2 μM plumbagin or 1 μM DPI for 24 hours. Arrows indicate the PH2A staining in the nuclei. Scale bar: 10 μm.
