Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells

Figure 3

Effect of serum presence on Nox4 localisation end nuclear ROS production. (a)  Representative images showing superimposing between DAPI (blue) and Nox4 SC (green) signals of AFSC cultures in the presence of absence of serum (FBS). Arrows indicate Nox4 staining in the nuclei. (b) Representative images of Western blot analysis revealed with Nox4 from Novus of cytosol and nuclei fractions of AFSC grown in the presence or absence (for 24 hours) of FBS. Actin and tubulin detection were performed in order to show the amount of protein loaded in each line and the nuclear fraction purity, respectively. Presented data are representative of three independent experiments. (c) Representative images showing staining with nuclear ROS probe (Nuclear peroxy Emerald 1) of AFSC with or without serum. Scale bar: 10 μm.