Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice: Analysis with Differential Acridine Orange/Ethidium Bromide Staining
Figure 2
(a) Fluorescent microphotograph of CD-1 mouse peripheral blood cells using the AO coating method (NCEs, PCEs, and MN-PCEs). (b) Morphology of viable cells (apoptotic and nonapoptotic cells) and nonviable cells (apoptotic and nonapoptotic cells) as assessed by acridine orange/ethidium bromide staining. Viable cells stain uniformly green (I), early apoptotic cells with intact plasma membranes appear green, with “dots” of condensed chromatin that are highly visible within (II), late apoptotic cells are stained bright green-orange because membrane blebbing starts to occur, EB can enter the cell (III), and apoptotic nonviable cells are stained bright orange because of the entry of ethidium bromide into these cells (VI).
