Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network

Figure 1

Build the H/R and P-PostH model. The culture media were replaced by glucose and serum free DMEM balanced in normal incubator in 30 minutes, and these HUVEC cells were then placed in hypoxic conditions which were created by a small enclosed humidified plexiglass chamber filled with 94% N₂, 5% CO₂, and 1% O₂ at 37°C for 12 h. After hypoxia, the medium was immediately washed off, and the HUVECs were returned to the maintenance medium (NCS-DMEM) in normal incubator for 4 h. At the same time, prepared propofol was added to the medium to different concentrations (25 μmol/L–150 μmol/L).