Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-κB Signaling Pathway

Figure 4

BPA suppressed C2C12 myoblast differentiation. Confluence C2C12 myoblast cells were cultured in differentiation conditions in the absence or presence of BPA for 72 h. Photomicrograph of immunofluorescence staining for MHC protein (green) and nuclei (blue) in differentiated myotubes (a). Total proteins were extracted and measured by BCA protein assay kit (b). Proteins were subjected to Western blot analysis with anti-MHC and anti-myogenin antibodies and expressed as relative band intensity to tubulin (c) and (d). Total RNA was extracted and subjected to real-time PCR with specific primers for the myogenic regulatory factors gene. Gene expression levels were expressed as fold change compared to the control group (e). ; ; compared to the control group. Scale bar = 200 µm.

(a)

(b)

(c)

(d)

(e)