Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-κB Signaling Pathway

Figure 3

BPA induced cellular apoptosis. C2C12 myoblast cells were cultured in the absence or presence of BPA at the indicated concentrations for 72 h. Photomicrographs of treated cells stained with Hoechst (blue) to visualize nuclear morphology (a). Total proteins were subjected to Western blot analysis with anti-caspase-3, anti-caspase-8, and anti-caspase-9 antibodies (b) and expressed as relative band intensity to tubulin (c). ; compared to the control group. Scale bar = 20 µm.

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(b)

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