Acute and Subchronic Toxicity Assessment of Conventional Soxhlet Cymbopogon citratus Leaves Extracts in Sprague–Dawley Rats

Figure 4

Representative micrographs of the spleen tissues in Sprague–Dawley rats after six weeks of CSE C. citratus ethanolic extract administration (x40). The micrographs show the white pulp (W) composed of lymphoid tissue with periarteriolar lymphoid sheaths (PALS) surrounding central arterioles (CA). Red pulp (R) regions show splenic cords composed of reticular fibers, macrophages, venus sinuses, and red blood cells. No significant inflammation, fibrosis, or abnormal cellularity was observed. (a) Spleen tissue of control rats treated with sterilized distilled water. (b) Spleen tissue of rats treated with 200 mg/kg bw.t CSE C. citratus ethanolic extract. (c) Spleen tissue of rats treated with 600 mg/kg bw.t CSE C. citratus ethanolic extract. (d) Spleen tissue of rats treated with 1200 mg/kg bw.t CSE C. citratus ethanolic extract.

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