Safety of Elixinol Hemp Extract: In Vitro Genetic Toxicity and Subchronic Toxicity in Rats

Table 2

Absolute organ weights () and relative organ to body weights (%) of 90-day study main study animals at termination (unless listed otherwise)^†.

Parameter	Control	28.94 mg/kg bw/day	50.64 mg/kg bw/day	86.81 mg/kg bw/day
Males
Terminal body weight (g)	516.0 ± 64.9	502.1 ± 75.3	536.1 ± 46.8	522.6 ± 90.1
Adrenals (g)	0.065 ± 0.011	0.055 ± 0.009	0.059 ± 0.010	0.058 ± 0.009
Adrenals/TBW	0.125 ± 0.014	0.111 ± 0.016	0.109 ± 0.017	0.114 ± 0.028
Brain (g)	2.273 ± 0.108	2.273 ± 0.084	2.306 ± 0.105	2.277 ± 0.154
Brain/TBW	4.447 ± 0.396	4.603 ± 0.584	4.320 ± 0.277	4.432 ± 0.525
Epididymides (g)	1.455 ± 0.179	1.444 ± 0.107	1.427 ± 0.190	1.432 ± 0.137
Epididymides/TBW	2.859 ± 0.483	2.930 ± 0.458	2.667 ± 0.317	2.800 ± 0.471
Heart (g)	1.487 ± 0.146	1.484 ± 0.251	1.476 ± 0.167	1.444 ± 0.214
Heart/TBW	2.902 ± 0.287	2.962 ± 0.339	2.755 ± 0.209	2.779 ± 0.240
Kidneys (g)	3.182 ± 0.301	3.367 ± 0.410	3.343 ± 0.320	3.592 ± 0.459
Kidneys/TBW	6.203 ± 0.510	6.777 ± 0.909	6.255 ± 0.568	6.948 ± 0.759
Liver (g)	13.289 ± 2.238	14.742 ± 2.425	14.795 ± 1.418	16.503 ± 2.507
Liver (g)-RC	14.726 ± 1.818	16.160 ± 2.051	15.026 ± 1.231	12.864 ± 1.524
Liver/TBW	25.653 ± 1.454	29.507 ± 3.993	27.623 ± 1.632	31.796 ± 2.819
Liver/TBW-RC	25.851 ± 0.874	26.093 ± 1.947	26.165 ± 1.249	24.990 ± 1.546
Liver/TBrW	5.825 ± 0.765	6.466 ± 0.885	6.410 ± 0.455	7.239 ± 0.847
Liver/TBrW-RC	6.243 ± 0.401	6.633 ± 0.854	6.224 ± 0.733	5.776 ± 0.708
Spleen (g)	0.927 ± 0.186	0.899 ± 0.173	0.890 ± 0.155	0.943 ± 0.188
Spleen/TBW	1.784 ± 0.195	1.795 ± 0.264	1.653 ± 0.194	1.868 ± 0.608
Testes (g)	3.781 ± 0.215	3.777 ± 0.221	3.768 ± 0.419	3.753 ± 0.285
Testes/TBW	7.432 ± 1.019	7.627 ± 0.834	7.050 ± 0.761	7.342 ± 1.187
Thymus (g)	0.255 ± 0.054	0.278 ± 0.095	0.232 ± 0.070	0.276 ± 0.094
Thymus/TBW	0.497 ± 0.110	0.552 ± 0.160	0.433 ± 0.122	0.524 ± 0.145
Females
Terminal body weight (g)	317.2 ± 34.3	304.7 ± 33.2	296.1 ± 21.1	297.6 ± 20.2
Adrenals (g)	0.081 ± 0.014	0.080 ± 0.009	0.080 ± 0.008	0.077 ± 0.016
Adrenals/TBW	0.257 ± 0.045	0.266 ± 0.032	0.270 ± 0.032	0.258 ± 0.051
Brain (g)	2.152 ± 0.058	2.157 ± 0.080	2.135 ± 0.136	2.142 ± 0.108
Brain/TBW	6.855 ± 0.752	7.140 ± 0.665	7.226 ± 0.450	7.219 ± 0.471
Heart (g)	1.053 ± 0.133	1.061 ± 0.120	1.008 ± 0.083	1.002 ± 0.055
Heart/TBW	3.320 ± 0.200	3.491 ± 0.287	3.414 ± 0.304	3.375 ± 0.217
Kidneys (g)	2.158 ± 0.219	2.171 ± 0.223	2.145 ± 0.215	2.255 ± 0.253
Kidneys/TBW	6.818 ± 0.383	7.154 ± 0.615	7.241 ± 0.486	7.594 ± 0.867
Liver (g)	9.327 ± 1.180	9.347 ± 1.467	9.804 ± 1.184	9.958 ± 0.696
Liver (g)-RC	8.328 ± 1.267	9.428 ± 0.846	10.020 ± 1.921	10.096 ± 0.752
Liver/TBW	29.360 ± 0.982	30.588 ± 2.199	33.120 ± 3.326	33.511 ± 2.089
Liver/TBW-RC	27.438 ± 2.664	30.158 ± 3.064	29.970 ± 3.019	31.238 ± 1.738
Liver/TBrW	4.336 ± 0.557	4.324 ± 0.577	4.593 ± 0.477	4.654 ± 0.330
Liver/TBrW-RC	3.926 ± 0.664	4.429 ± 0.534	4.693 ± 0.772	4.737 ± 0.435
Ovaries (g)	0.134 ± 0.024	0.135 ± 0.020	0.141 ± 0.014	0.140 ± 0.018
Ovaries/TBW	0.424 ± 0.070	0.447 ± 0.068	0.477 ± 0.040	0.470 ± 0.057
Spleen (g)	0.582 ± 0.084	0.621 ± 0.081	0.589 ± 0.077	0.658 ± 0.056
Spleen/TBW	1.835 ± 0.190	2.042 ± 0.213	1.987 ± 0.183	2.216 ± 0.189
Thymus (g)	0.274 ± 0.069	0.257 ± 0.098	0.306 ± 0.096	0.270 ± 0.041
Thymus/TBW	0.862 ± 0.176	0.842 ± 0.300	1.035 ± 0.325	0.913 ± 0.162
Uterus (g)	0.794 ± 0.218	0.881 ± 0.311	0.844 ± 0.257	0.807 ± 0.211
Uterus/TBW	2.517 ± 0.723	2.873 ± 0.917	2.898 ± 1.077	2.703 ± 0.631

N = 10/group for the main study and N = 5/group for recovery groups. Data are presented as mean ± standard deviation (SD). Relative organ weights (ratios) presented in the table are times 1000. ^†Data for organ weight as a % of brain weight, as well as data for recovery group animals are included in the table if absolute organ weight or organ weight as a % of body weight in main study animals was affected. Significantly different from control by Dunnett’s or Dunn’s test (2-sided) (); Significantly different from control by Dunnett’s or Dunn’s test (2-sided) (); Significantly different from control by Dunnett’s or Dunn’s test (2-sided) (). bw = body weight; g = grams; kg = kilogram; mg = milligrams; RC = recovery group; TBW = terminal body weight; TBrW = terminal brain weight.