Toxicological Evaluation of a Mixture of Astragalus membranaceus and Panax notoginseng Root Extracts (InnoSlim®)

Table 1

Summary of the results of the initial mutation test.

Initial Mutation Test (Plate Incorporation Test)
Concentrations (μg/plate)	Salmonella typhimurium tester strains																Escherichia coli
	TA 98				TA 100				TA 1535				TA 1537				WP2uvrA
	-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9
Mean values of revertants per plate Mutation rate (MR)	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR
Untreated Control	15.7	1.09	15.3	0.82	62.3	0.88	77.7	0.77	8.3	0.89	9.7	1.21	7.7	0.92	6.0	1.06	34.3	0.75	48.3	1.06
DMSO Control	13.7	1.00	16.0	1.00	–	–	92.0	1.00	–	–	9.7	1.00	6.0	1.00	7.0	1.00	–	–	44.0	1.00
Ultrapure Water Control	14.3	1.00	18.7	1.00	70.7	1.00	100.7	1.00	9.3	1.00	8.0	1.00	8.3	1.00	5.7	1.00	46.0	1.00	45.7	1.00
TEST ITEM
5000	16.3	1.14	24.3	1.30	73.7	1.04	89.7	0.89	9.3	1.00	8.0	1.00	9.0	1.08	7.3	1.29	37.7	0.82	44.3	0.97
1600	14.0	0.98	19.0	1.02	68.3	0.97	96.7	0.96	10.0	1.07	9.0	1.13	7.0	0.84	4.0	0.71	42.0	0.91	54.3	1.19
500	13.7	0.95	23.3	1.25	85.3	1.21	108.0	1.07	10.3	1.11	10.7	1.33	9.0	1.08	4.7	0.82	48.7	1.06	47.3	1.04
160	16.7	1.16	18.7	1.00	79.3	1.12	113.7	1.13	11.0	1.18	11.0	1.38	5.7	0.68	5.0	0.88	44.7	0.97	51.0	1.12
50	11.3	0.79	17.0	0.91	68.7	0.97	101.3	1.01	10.3	1.11	11.7	1.46	7.3	0.88	11.3	2.00	40.0	0.87	44.3	0.97
16	19.7	1.37	25.0	1.34	72.7	1.03	101.7	1.01	10.7	1.14	11.0	1.38	7.0	0.84	6.7	1.18	35.7	0.78	55.0	1.20
POSITIVE CONTROLS
NPD (4 μg/plate)	387.3	28.34	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SAZ (2 μg/plate)	–	–	–	–	848.0	12.00	–	–	853.3	91.43	–	–	–	–	–	–	–	–	–	–
9AA (50 μg/plate)	–	–	–	–	–	–	–	–	–	–	–	–	245.3	40.89	–	–	–	–	–	–
MMS (2 μL/plate)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	1053.3	22.90	–	–
2AA (2 μg/plate)	–	–	1757.3	109.83	–	–	2077.3	22.58	–	–	171.3	17.72	–	–	130.7	18.67	–	–	–	–
2AA (50 μg/plate)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	241.0	5.48

MR, mutation rate; DMSO, dimethyl sulfoxide; NPD, 4-Nitro-1,2-phenylenediamine; SAZ, sodium azide; 9AA, 9-aminoacridine; MMS, methyl methanesulfonate; 2AA, 2-aminoanthracene.
Remarks. Ultrapure water was applied as vehicle of the test item and the positive control substances: SAZ and MMS; and the DMSO was applied as vehicle for positive control substances: NPD, 9AA, and 2AA. The mutation rate of the test item, SAZ, MMS, and untreated control refers to the ultrapure water; the mutation rate of NPD, 9AA, and 2AA refers to DMSO.