Lyophilized B. subtilis ZB183 Spores: 90-Day Repeat Dose Oral (Gavage) Toxicity Study in Wistar Rats
Table 3
Summary of significant haematology and coagulation parameters.
Dose group (mg/kg/day)
Males
Females
Control
20/10
200/100
2000/1000
Control
20/10
200/100
2000/1000
RBC (10^12/L)
8.94 ± 0.36
8.64 ± 0.29
9.00 ± 0.47
8.83 ± 0.34
8.21 ± 0.24
8.41 ± 0.34
8.10 ± 0.28
8.23 ± 0.31
HGB (g/L)
150 ± 5
146 ± 2
150 ± 6
149 ± 4
149 ± 5
149 ± 5
148 ± 4
149 ± 5
HCT (L/L)
0.467 ± 0.015
0.454 ± 0.015
0.468 ± 0.021
0.464 ± 0.012
0.458 ± 0.012
0.461 ± 0.015
0.456 ± 0.016
0.459 ± 0.013
MCV (fL)
52.2 ± 1.2
52.6 ± 1.7
52.0 ± 1.8
52.6 ± 1.3
55.8 ± 1.1
54.0 ± 1.5
56.2 ± 2.0
55.8 ± 1.3
MCH (pg)
16.8 ± 0.6
17.0 ± 0.7
16.7 ± 0.6
16.0 ± 0.6
18.2 ± 0.5
17.7 ± 0.6
18.3 ± 0.7
18.1 ± 0.5
MCHC (g/L)
323 ± 6
323 ± 9
321 ± 4
321 ± 7
325 ± 3
323 ± 6
325 ± 7
325 ± 5
Retic A (10^12/L)
0.183 ± 0.035
0.201 ± 0.022
0.205 ± 0.022
0.193 ± 0.032
0.198 ± 0.030
0.196 ± 0.034
0.200 ± 0.027
0.180 ± 0.025
Retic (%)
2.05 ± 0.37
2.32 ± 0.22
2.28 ± 0.23
2.20 ± 0.40
2.42 ± 0.41
2.34 ± 0.42
2.47 ± 0.34
2.18 ± 0.28
RDW (%)
13.2 ± 0.4
14.0 ± 1.4
13.5 ± 0.6
13.4 ± 0.4
11.7 ± 0.4
11.9 ± 0.4
11.9 ± 0.5
11.6 ± 0.4
HDW (g/L)
25.2 ± 1.0
27.1 ± 4.2
26.5 ± 1.2
24.6 ± 0.9
21.1 ± 1.0
21.3 ± 0.6
21.4 ± 0.4
20.6 ± 0.6
Hyper (%)
2.9 ± 0.7
3.2 ± 2.0
2.9 ± 0.6
∗∗1.7 ± 0.4
1.2 ± 0.3
1.1 ± 0.5
1.2 ± 0.7
1.0 ± 0.3
Hypo (%)
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
Macro (%)
0.0 ± 0.0
0.1 ± 0.2
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
Micro (%)
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
RBC Fragments (10^12/L)
0.08 ± 0.01
0.09 ± 0.01
0.09 ± 0.01
0.09 ± 0.01
0.07 ± 0.02
0.08 ± 0.01
0.07 ± 0.01
0.08 ± 0.02
RBC ghosts (10^12/L)
0.04 ± 0.02
0.03 ± 0.01
0.03 ± 0.01
0.03 ± 0.01
0.03 ± 0.00
0.03 ± 0.01
0.04 ± 0.01
0.03 ± 0.01
PLT (10^9/L)
962 ± 88
972 ± 74
1010 ± 138
1037 ± 61
938 ± 135
984 ± 90
945 ± 62
1027 ± 101
WBC (10^9/L)
6.50 ± 0.89
6.59 ± 1.43
6.42 ± 1.28
5.53 ± 1.85
3.58 ± 1.12
3.77 ± 0.79
3.50 ± 0.62
4.34 ± 1.14
Neut A (10^9/L)
1.15 ± 0.36
1.15 ± 0.24
1.40 ± 0.44
0.93 ± 0.33
0.76 ± 0.23
0.40 ± 0.37
0.75 ± 0.26
0.72 ± 0.16
Lymp A (10^9/L)
5.05 ± 0.68
5.10 ± 1.59
4.61 ± 1.04
4.34 ± 1.49
2.59 ± 0.98
2.50 ± 0.58
2.56 ± 0.54
3.43 ± 1.02
Mono A (10^9/L)
0.22 ± 0.07
0.24 ± 0.07
0.29 ± 0.13
0.18 ± 0.07
0.13 ± 0.03
0.15 ± 0.05
0.11 ± 0.05
0.10 ± 0.04
Baso A (10^9/L)
0.01 ± 0.00
0.01 ± 0.01
0.01 ± 0.01
0.01 ± 0.01
0.00 ± 0.00
0.00 ± 0.00
0.01 ± 0.01
∗0.01 ± 0.00
Eosi A (10^9/L)
0.07 ± 0.03
0.07 ± 0.03
0.10 ± 0.04
0.08 ± 0.04
0.09 ± 0.05
0.11 ± 0.09
0.07 ± 0.04
0.08 ± 0.03
Prothrombin time (seconds)
17.1 ± 0.2
16.5 ± 0.4
∗∗15.7 ± 0.6
17.0 ± 0.9
16.1 ± 0.7
16.1 ± 0.7
15.8 ± 0.5
∗∗15.4 ± 0.5
APTT (seconds)
15.8 ± 1.6
∗∗11.8 ± 2.3
∗∗13.4 ± 1.7
13.8 ± 1.8
15.2 ± 2.5
13.1 ± 1.5
15.1 ± 1.8
14.1 ± 1.8
Values are expressed as mean ± standard deviation. mg/kg, milligrams per kilogram body weight per day; L, liter; n, number of animals; ∗/∗∗ Significantly higher/lower than the vehicle control group at .
