Lyophilized B. subtilis ZB183 Spores: 90-Day Repeat Dose Oral (Gavage) Toxicity Study in Wistar Rats
Table 2
Summary of significant functional observation battery results.
Control
Dose group (mg/kg/day)
Males
20/10
200/100
2000/1000
Physiological observation
Body temperature (°C)
37.02
37.05
37.2
37.09
Body weight (g)
457.31
482.39
453.33
483.39
Hindlimb’s footsplay (mm)
84.07 ± 5.72
92.4 ± 16.43
∗∗68.73 ± 16.53
81.03 ± 13.08
Motor activity score
Stereotypic time (secs)
Interval 1
96 ± 20.06
97.1 ± 16.76
112.1 ± 8.89
107.5 ± 17.93
Interval 2
125.5 ± 26.12
118.9 ± 24.08
115.6 ± 27.92
96.8 ± 24.16
Interval 3
99.2 ± 41.89
97.6 ± 34.57
104.9 ± 51.32
73 ± 37.9
Total
320.7 ± 66.82
313.6 ± 55.79
332.6 ± 63.15
277.3 ± 52.69
Ambulatory time (secs)
Interval 1
363.6 ± 31.83
403.7 ± 35.74
367 ± 39.65
347.2 ± 57.29
Interval 2
250.6 ± 55.48
257.4 ± 52.4
245.1 ± 53.95
∗∗183.30 ± 73.17
Interval 3
186.2 ± 46.78
197.1 ± 56.33
143.8 ± 64.99
142.3 ± 60.25
Total
800.4 ± 108.78
858.2 ± 131.3
755.9 ± 127.24
672.8 ± 149.45
Horizontal counts
Interval 1
3657.6 ± 653.47
4009.4 ± 935.8
3628.8 ± 1207.59
3065.9 ± 965.67
Interval 2
2203.3 ± 550.4
2073.5 ± 551.74
2041.6 ± 838.06
∗∗1474.50 ± 815.27
Interval 3
1436.1 ± 481.53
1445.8 ± 499.94
1163.5 ± 563.2
1090.6 ± 591.75
Total
7297 ± 1459.22
7528.7 ± 1877.99
6833.9 ± 2376.54
5631 ± 2062.82
Hindlimb’s grip strength
Average
662.67 ± 7.6
660.53 ± 12.47
664.27 ± 16.04
657.93 ± 16.77
Values are expressed as mean ± standard deviation. g: grams; mm: millimeters; secs: seconds; ∗∗ : Significantly lower than the vehicle control group at .
