Research Article
Lyophilized B. subtilis ZB183 Spores: 90-Day Repeat Dose Oral (Gavage) Toxicity Study in Wistar Rats
Table 1
Details of CFU/g for both lot numbers of test item, doses administered and treatment days.
| | Test item lot no. | CFU (billion/g) | Calculated CFU | Group | Dose (mg/kg/day) | Dose volume (mL/kg) | Concentration (mg/mL) | Treatment period | | NA | NA | NA | Vehicle control (G1) | 0 | 10 | 0 | Days 1–90 |
| | 300L engineering batch ZBT-002a | 58.5 | ~109 CFU | Low dose (G2) | 20 | 10 | 2 | Days 1–66 | | ~1010 CFU | Mid dose (G3) | 200 | 10 | 20 | | ~1011 CFU | High dose (G4) | 2000 | 10 | 200 |
| | 300L engineering batch ZBT-002a part 2 | 135 | ~109 CFU | Low dose (G2) | 10 | 10 | 1 | Days 67–90 | | ~1010 CFU | Mid dose (G3) | 100 | 10 | 10 | | ~1011 CFU | High dose (G4) | 1000 | 10 | 100 |
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