An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 9
(a) Summary of food consumption in the 90-day repeated oral toxicity study
Group (mg/kg bw/day)
Daily mean food consumption (g/animal/d) on weeks
1
2
3
4
5
6
7
8
9
10
11
12
13
Males
Control
20.2 ± 1.60
21.1 ± 1.68
23.1 ± 1.81
22.0 ± 1.50
20.3 ± 1.51
20.4 ± 2.04
18.8 ± 1.62
19.6 ± 1.55
19.4 ± 1.28
17.8 ± 1.60
19.8 ± 1.72
18.0 ± 1.83
16.1 ± 1.52
100
20.5 ± 1.68
20.3 ± 2.98
22.8 ± 3.34
22.1 ± 3.37
20.6 ± 2.74
19.8 ± 2.49
18.7 ± 2.59
17.4 ± 2.09∗∗
18.1 ± 1.96
16.8 ± 2.02
18.0 ± 1.94
16.9 ± 1.94
15.3 ± 1.63
360
16.7 ± 1.64∗∗
17.9 ± 1.18∗∗
20.1 ± 1.03∗∗
19.7 ± 1.35∗∗
18.4 ± 1.61
17.9 ± 2.23
16.9 ± 2.23
15.5 ± 1.78∗∗
16.9 ± 1.66∗∗
16.4 ± 1.68
16.7 ± 1.87∗∗
16.2 ± 2.32
14.0 ± 1.45∗∗
720
14.6 ± 1.17∗∗
16.9 ± 1.19∗∗
18.9 ± 1.21∗∗
18.6 ± 0.81∗∗
17.8 ± 0.93∗∗
17.5 ± 0.82∗∗
16.3 ± 1.29∗∗
15.8 ± 1.09∗∗
16.6 ± 0.91∗∗
16.3 ± 1.16
16.6 ± 1.14∗∗
15.9 ± 1.39∗∗
13.8 ± 1.36∗∗
Females
Control
14.3 ± 1.00
13.5 ± 1.07
16.2 ± 1.39
15.8 ± 1.93
15.0 ± 2.64
15.7 ± 2.11
15.0 ± 2.04
15.6 ± 2.17
16.7 ± 2.22
14.6 ± 1.82
16.0 ± 1.95
15.0 ± 1.98
13.6 ± 1.65
100
14.2 ± 1.58
13.3 ± 1.33
14.9 ± 1.36
15.1 ± 1.38
14.3 ± 1.66
14.0 ± 1.24∗∗
13.5 ± 1.77
12.9 ± 1.32∗∗
14.2 ± 1.43∗∗
13.5 ± 1.81
14.3 ± 1.86
13.6 ± 2.07
12.3 ± 2.07
360
11.7 ± 0.81∗∗
11.8 ± 0.92∗∗
14.1 ± 0.88∗∗
14.2 ± 0.83
13.6 ± 0.97
13.1 ± 1.27∗∗
12.3 ± 1.23∗∗
11.9 ± 1.37∗∗
13.5 ± 1.03∗∗
11.7 ± 1.56∗∗
12.6 ± 1.63∗∗
11.9 ± 1.83∗∗
10.5 ± 1.84∗∗
720
10.4 ± 1.28∗∗
11.5 ± 1.23∗∗
14.0 ± 0.95∗∗
14.1 ± 1.15∗∗
13.1 ± 1.24
12.7 ± 1.11∗∗
12.0 ± 1.27∗∗
11.3 ± 1.22∗∗
12.5 ± 0.92∗∗
11.3 ± 1.16∗∗
12.0 ± 1.22∗∗
11.7 ± 1.26∗∗
10.1 ± 1.31∗∗
= 15 for control and 720 mg/kg bw/day groups and = 10 for all other groups. p < 0.05, ∗∗p < 0.01, statistical significances were determined with Duncan’s multiple range test or with Mann–Whitney test versus control.
(b) Summary of food consumption in the recovery period
Group (mg/kg bw/day)
Mean food consumption (g/animal/d) on weeks
1
2
3
4
Males
Control
23.9 ± 1.2
25.5 ± 1.2
24.5 ± 1.1
23.9 ± 1.0
720
19.7 ± 1.7∗∗
24.2 ± 1.4
22.6 ± 1.8
22.7 ± 2.0
Females
Control
15.9 ± 1.1
17.9 ± 1.7
16.2 ± 1.1
16.5 ± 1.1
720
12.8 ± 1.3∗∗
15.4 ± 1.8
13.5 ± 2.5
14.9 ± 0.9
= 5 for all groups. p < 0.05, ∗∗p < 0.01, statistical significances were determined with Duncan’s multiple range test or with Mann–Whitney test versus control.
