Estimation of the Mechanism of Adrenal Action of Endocrine-Disrupting Compounds Using a Computational Model of Adrenal Steroidogenesis in NCI-H295R Cells

Figure 4

Comparison of time-course profiles of cholesterol and adrenal steroids produced by NCI-H295R cells between experimentally measured and simulated data. To intuitively confirm reconstruction of the measured experimental data in the developed simulation model of NCI-H295R cells, dynamics of cholesterol and adrenal steroids produced by NCI-H295R cells were plotted to overlay experimental data with the simulated results. Graphs show the dynamics of medium concentrations of cholesterol (a) and adrenal steroids ((b)–(d)) and intracellular concentrations of cholesterol (e) and adrenal steroids ((f)–(h)). Steroid hormones were categorized into three groups by concentration levels. Major steroids were PREG: pregnenolone, DCORTICO: 11-deoxycorticosterone, DCORT: 11-deoxycortisol, CORTICO: corticosterone, and CORT: cortisol ((b) and (f)). Moderate steroids were HPREG: 17α-hydroxypregnenolone, PROG: progesterone, HPROG: 17α-hydroxyprogesterone, DIONE: androstenedione, and E1: estrone ((c) and (g)). Minor steroids were DHEA: dehydroepiandrosterone, ALDO: aldosterone, TESTO: testosterone, and E2: 17β-estradiol ((d) and (h)). Experimental data are shown as symbols with dotted lines. All data represent the mean ± SD (). Simulation data are shown as solid lines.

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