Research Article
Increased Susceptibility to Ethylmercury-Induced Mitochondrial Dysfunction in a Subset of Autism Lymphoblastoid Cell Lines
Figure 2
No residual NAC remains in pretreated cells following washing regimen. HPLC chromatograms from a standard preparation of NAC (a) and two million AD LCLs pretreated with NAC and washed two times with DMEM XF assay media (b). The NAC peak seen at retention time 3.71 min in the standard preparation (a) is not detectable in the cell extract, whereas the GSH peak at 4.36 min is prominent (b).
| (a) |
| (b) |