Circadian Rhythms and Breast Cancer: The Role of Per2 in Doxorubicin-Induced Cell Death

Figure 6

Determination of G2/M cell cycle transition in MDA-MB-231 breast cancer cells following Per2 silencing and doxorubicin treatment. MDA-MB-231 cells were subjected to (1) control conditions (C), (2) 2.5 μM doxorubicin (D), (3) 30 nM Per2 esiRNA (P), and (4) 30 nM Per2 for 48 hours + 2.5 μM doxorubicin for 24 hours (PD). G2/M transition analysis was assessed by flow cytometry. Statistical analysis: one way ANOVA with Bonferroni post hoc correction. All results are presented as mean ± SEM (). versus control, versus control, and versus control.