High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR

Figure 4

Rapamycin decreases high-glucose injury in HK-2 cells.(a)Flow cytometry and quantification to assay apoptosis of HK-2 cells under the NG, HG, HG plus treatment with a plasmid expressing an shRNA against Netrin-1 (shNetrin-1), HG plus rapamycin (a mTOR inhibitor) and HG+Netrin-1+Rapa. (b) Western blotting analyses of Bcl-2, Bax and cleaved caspase-3 in HK-2 cells. Equal protein loading was confirmed using an anti-GAPDH antibody; Band intensity of Bcl-2, Bax and cleaved caspase-3. (c) Fluorescein—phalloidin staining of actin fibers with indicated treatments and transfections (scale  μm). Values are . , , and . vs. HG group: , , and . vs.HG+Rapa group: , , and .

(a)

(b)

(c)