High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR

Figure 2

Overexpression of Netrin-1 mediated the Akt/mTOR pathway and induced autophagy. (a) Western blotting analyses of p-mTOR, mTOR, p-Akt, Akt, LC3, P62, and Beclin-1 in HK-2 cells under the NG or HG; Band intensities of p-mTOR/ mTOR, p-Akt/ Akt, LC-3Ⅱ/Ⅰ, P62 and Beclin-1. (b) Adenovirus encoding GFP-RFP-LC3 were transiently transfected into cells followed by treating with NG (5.5mM glucose), HG (30.0mM glucose) or HG+Netrin-1(30.0mM glucose+20ng/ml recombinant Netrin-1) for 24h, respectively. The intensity of GFP and RFP puncta was gauged by Image J program. Scale bars were 10 μm. (c) Electron microscopy analyses number of autophagosome (original magnification and ~1,200x) in different group. Values are . vs. LG group: , , and . vs. HG group: , , and .

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