High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR

Figure 1

Overexpression of Netrin-1 ameliorated hyperglycemia-induced apoptosis and actin cytoskeleton derangement. (a) Western blotting analyses of Netrin-1 level in HK-2 cells under 30.0mMglucose treatment (HG group) compared with the control group (5.5mM glucose treatment, NG group); Band intensity of Netrin-1. (b) Western blotting analyses of Bcl-2, Bax and cleaved caspase-3 in HK-2 cells under the NG or HG. Equal protein loading was confirmed using an anti-GAPDH antibody; Band intensity of Bcl-2, Bax and cleaved caspase-3. (c) Flow cytometry analysis of apoptotic HK-2 cells under the NG (control), HG or HG plus treatment with recombinant Netrin-1 condition. (d) Fluorescein—phalloidin staining of actin fibers in cultured HK-2 cells. Actin cytoskeleton derangement was reduced in HK-2 cells under the HG plus treatment with recombinant Netrin-1 condition (scale  μm). All experiments were conducted in triplicate. Values are . vs. LG group: , , and . vs. HG group: , , , , , .

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