Quercetin Inhibits Pyroptosis in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 2

Quercetin ameliorates high-glucose-induced cardiomyocyte injury. (a) CCK-8 revealed changes in cell viability after coincubating H9C2 cells with different concentrations of quercetin for 24 h. (b) Expression levels of pyroptosis-related proteins detected by WB. Data are expressed as the , , H-G vs. Con-G, and Que-G. (c) The expression level of NLRP3 in cardiomyocytes from each group detected by immunofluorescence ( magnification). (d) Fluorescence detection of mitochondrial membrane potential. The brighter the red fluorescence, the stronger the mitochondrial membrane potential; the opposite condition stands for green fluorescence ( magnification). (e) A ROS fluorescent probe was used to detect the accumulation of ROS in cardiomyocytes in each group ( magnification). CON-G: glucose concentration of 5.5 mmol/L; H-G: glucose concentration of 30 mmol/L; Que-G: glucose concentration of 30 mmol/L+quercetin; ROS: reactive oxygen species.

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