Quercetin Inhibits Pyroptosis in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 1

Quercetin ameliorates myocardial fibrosis in diabetic rats (a) ELISA assays for detecting the expression levels of NF-κB, IL-1, TGF-α, and BNP in the serum of rats in each group. (b) Expression levels of pyroptosis pathway-associated proteins in the myocardial tissue of rats in each group detected by western blotting (WB). (c) H&E and Masson’s trichrome staining of rat myocardial tissues, and immunofluorescence detection of the expression of collagen II in myocardial tissues ( magnification). Quantitative analysis of the expression of collagen II in Masson’s-stained sections and NLRP3 immunohistochemistry using ImageJ. (d) TUNEL assay detecting the apoptotic levels of myocardial tissue cells in each group ( magnification). (e) Expression levels of Nrf2 in the nucleus and those of HO-1 and GCLC in the cell were detected by WB. H3: histone H3, used as a reference nuclear protein for normalization. CON-Group: control group; DM-Group: diabetic group; Qod-Group: interval dosing+diabetic group; Qd-Group: daily dosing and diabetic group; WB: western blotting. Data are expressed as the , , DM-G vs. Con-G, and Qod-G. ^#, Qod-G vs. Qd-G. ^{^}, Qod-G vs. Con-G, and DM-G.

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