Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGF/ERK/FAK/Src Pathway Signaling

Figure 5

SCU targeted VEGF to attenuate hyperglycemia-induced tubulogenesis. (a) The migration of HRECs and HRECs^kd-VEGF treated with glucose+CoCl₂ was measured by transwell migration assays and wound healing assays. (b) The quantitative results of HREC transwell experiment were measured by a microplate reader. (c) Scratched areas, which were not covered by migrated cells, were measured using ImageJ (NIH) for the quantification. Data in (b) and (c) were presented as (); compared to the control group and the glucose+CoCl₂+siVEGF group. ^# compared to the glucose+CoCl₂ group and the glucose+CoCl₂+siControl group. (d) Western blot analysis of p-FAK, p-Src, and p-ERK in HRECs and HRECs^kd-VEGF treated with glucose+CoCl₂. (e) The migration of HRECs and HRECs^OE-VEGF treated with glucose+CoCl₂+SCU was measured by transwell migration assays and wound healing assays. (f) The quantitative results of HREC transwell experiment were measured by a microplate reader. (g) Scratched areas, which were not covered by migrated cells, were measured using ImageJ (NIH) for the quantification. Data in (f) and (g) were presented as (); compared to the control group, the glucose+CoCl₂+SCU group, and the glucose+CoCl₂+SCU+scramble group. ^# compared to HRECs^OE-VEGF with the SCU glucose+CoCl₂. (h) Western blot analysis of p-FAK, p-Src, and p-ERK in HRECs and HRECs^OE-VEGF treated with glucose+CoCl₂+SCU.

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