Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice

Figure 7

Blood flow of sciatic nerves and plantar skins were ameliorated by transplantation of angioblast-like cell derived from ES cells (ES-ABs). At a time point of 12 weeks of diabetes (before treatment), blood flows of plantar skins (a) and sciatic nerves (b) in diabetic mice significantly decreased compared with those in normal mice, and, 4 weeks after transplantation (after treatment), the decreases were ameliorated in transplanted limbs of diabetic mice. However, administration of ES-ABs did not alter blood flow in those of normal mice. N: normal mice limbs, DM: diabetic mice limbs, S: saline-injected limbs, AB+: limbs transplanted ES-ABs, and AB−: contralateral limbs transplanted ES-ABs. Results are means ± SD. versus pretreatment N. versus posttreatment S-treated N, versus posttreatment S-treated DM. –6 in each pretreatment group and –10 in each posttreatment group.

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(b)