Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice

Figure 4

Tube forming assay of angioblast-like cells induced from ES cells (ES-ABs) on Matrigel. To elucidate whether ES-ABs themselves are enabled to form blood vessels, sorted ES-ABs and Flk1 negative cells were, respectively, seeded on Matrigel. Two days later, cells were visualized with isolectin IB4, a stain of endothelial cell. (a) Although endothelial cells stained with IB4 were rarely induced from Flk1 negative cells (right), heavy numbers of endothelial cells were induced from ES-ABs and deployed cobblestone-like pattern (left). Bar: 100 μm. Red: isolectin IB4. (b) In ES-AB culture, a part of cells stained with IB4 structured vessel like tube formation (left 2 images). On the other hand, cells forming a line were not stained with IB4 in culture of Flk1 negative cells (right 2 images). Bar: 100 μm. Red: isolectin IB4 and blue: DAPI.

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