Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters

Figure 7

AOA treatment reduces biofilm-forming microbiota and increases bacterial antibiotic susceptibility: (a) The bacterial contents of the swab samples were evaluated by measuring optical densities at OD 570 nm. AOA treatment reduced biofilm formation. (b) Individual bacterial colonies with biofilm-forming-capacity were quantified by measuring the optical densities and were also reduced with AOA treatment. (c) Bacterial prevalence of individual species was again reduced with AOA treatment. (d) Bacterial burden measured by colony forming unit counts was significantly reduced in AOA treated db/db wounds. (e) Community minimum inhibitory concentration (CMIC) of bacteria present in wounds treated with AOA was performed using gentamicin (Gm), carbenicillin (Cb), and amoxicillin (Amox). CMIC degreased significantly with time of AOA treatment. . All data are mean ± SD. , . = nonsignificant.

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